Products : SpectraMax M5/M5e reader Fluorescence-based mitochondrial function analysis (PDF) Summary: Mitochondrial dysfunction is implicated in numerous disease states and is also a major mechanism of drug-induced toxicity. 0112-0115G October2018 SpectraMax®M3 SpectraMax®M4 SpectraMax®M5 SpectraMax®M5e Multi-ModeMicroplateReaders UserGuideSpectraMax® Quant™ dsDNA Assay Kits The SpectraMax® Quant™ dsDNA Assay Kits are designed for fluorescence-based dsDNA quantitation across a broad range of concentrations. Simultaneous dual emission detection enables read times of only 2:17 for an entire 384-well plate. A neurosurgeon from Mercer Island and a noted mountaineer from Spokane, Washington, were killed on February 4, 1986, when. Accurate quantitation The optical design of the SpectraMax 340PC384 reader truly mimics a dual. 8 x 10-15. 8 x 10-15. This application note describes the use of the SPECTRAmax ® GEMINI XS from Molecular Devices to study the performance of the LIVE/DEAD ® Viability/Cytoxicity Assay Kit for animal cells. 01) + 0. Each tube of fluorophore also contained ROX. Plate chamber design of the SpectraMax category 1 microplate readers. 2 x 10-14. Gemini EM. Jeffrey Manber, Managing Director of NanoRacks said, "Having the SpectraMax M5e Reader on-orbit will allow researchers to undertake research with real-time results. KEY FEATURES Molecular Devices SpectraMax Plus 384 UV/Vis Microplate Reader. The SpectraMax ® M3, M4, M5, and M5 e Microplate Readers are a series of dual-monochromator, multidetection, multi-mode instruments with a triple-mode cuvette port and 6-well to 384-well microplate reading SpectraMax i3 Install Guide; SpectraMax i3; SpectraMax i3x; SpectraMax iD3; SpectraMax iD5; SpectraMax Mini; SpectraMax Mini Install Guide; SpectraMax Mini Reader Plug-In Download Page (required for SoftMax Pro 7. FlexStation II 384. This integrated system provides users with a multi-detection platform capable of increasing the liquid handling throughput and flexibility for biochemical- and cell-based kinetic assays. Detection cartridge modularity allows for easy addition of new read modes within minutes without. 0. Dual monochromators for assay flexibility. FlexStation™ III. Molecular Devices SpectraMax® M5/M5e Microplate Reader Have A Question? Contact Our Technical Support Team NA: 800-955-6288 Or INTL: 760-603-7200 Ext. 1. The SpectraMax M5e Micoplate Reader is the standard for UV/Visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96-well and 384-well microplates. HTRF is compatible with a large range of available microplate readers. 1. The SpectraMax M5e reader offers the additional benefit of being certified for Cisbio Bioassays HTRF® technology. 00 USD. The SpectraMax M5e will debut today at the American Association for Cancer Research Annual Meeting in Washington, D. 1) SpectraMax Glo Steady Luc Reporter Assay (6. 6 M HCl for 24 h. M5 reader microplate m3 spectramax molecular devices llc m5e biocompare quote fluorescenceMolecular devices spectramax plus 384 user manual pdf download Spectramax® m2/m2e multimode microplate readers, with softmax® proMolecular devices spectramax m5 microplate reader labx contract svc warranty available manufacturer. Table of Contents. 2 x 10-14. The guidelines for cuvette use in SpectraMax instruments are the same as those that apply to any high-quality spectrophotometer. Join. No special upgrade is required for HTRF ® readout, as it is a monochromator-based instrument. 3. The SpectraMax® M5e includes htrf certification, and both the M5 and M5e are lanthascreen certified. StakMax Handling Stacker Base Unit. AquaMax® 4000. These were dispensed directly into a microplate and did not require any thermal cycling. 3 or higher) SpectraMax® M5 and M5e Multi-Mode Microplate Readers; SpectraMax® M4 Multi-Mode Microplate Reader; SpectraMax® M3 Multi-Mode Microplate Reader; SpectraMax® M2 and M2e Multi-Mode Microplate Readers; SpectraMax® 340PC 384 Absorbance Microplate ReaderMolecular Devices SpectraMax M5/M5e Microplate Reader was tested for compatibility with Life Technologies LanthaScreen® Kinase Binding and Adapta™ Europium-based TR-FRET assays. 5 mP standard deviation at 1 nMMolecular Devices SpectraMax M5e Microplate Reader $26,500. SpectraMax M2, M2e; SpectraMax M3, M4, M5, M5e; FilterMax™ Multi-Mode Detection. 1. 1)M5e = 174nM SpectraMax M5e delta F % Paradigm = 18nM M5e = 19nM Figure 1. assays on the SpectraMax® Paradigm® microplate detection platform. The Analyst®, FlexStation® and SpectraMax® M5/M5e from Molecular Devices have received the LanthaScreen® Certified designation from Invitrogen™ which ensures that these readers are validated to strict standards in instrument seFigure 2: Expression of mCherry with (improved BBa_K1141000) and without (BBa_K1141000) the translation enhancing 5’ untranslated region was measured (Ex: 587 nm, Em: 610 nm) over a period of 6 hours after induction with 0. ZERO BIAS - scores, article reviews, protocol conditions and more-Dual monochromatic optics -Wide range of applications -Top or bottom read modes The SpectraMax M3 Multi-Mode Microplate Reader provides three modes of detection in one platform The SpectraMax M3 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range. 5 mP standard deviation at 1 nM SpectraMax M3, M4, M5, and M5 e Multi-Mode Microplate Readers 0112-0115 F 9 PathCheck ® Pathlength Meas urement Technology A SpectraMax Multi-Mode Microplate Reader with PathCheck Pathlength Measurement Technology allows normalization of variable well volumes to 1-cm cuvette readings. SpectraMax® M3, M4, M5, and M5e Multi-Mode Microplate Readers User Guide 0112-0115 F July 2010 M3-M5e_manual. A typical HTRF assay uses a europium cryptate as the donor, and the organic fluorophore d2 as the acceptor. The dual monochromator optics allow. With optimized reagents, option to operate the readers. 00 - Original price $5,513. SpectraMax Absorbance, Fluorescence, Luminescence, and Multi-Mode Readers. After excluding the background values, the cross‐reactivity of the. The assay was set up in a low-volume 384- well black plate using the pipetting. 1. S. Absorbance at a wavelength of 450 nm was measured using the SpectraMax M5e multi-mode microplate reader (Molecular Devices, San Jose, CA, USA) to quantify cytokine. Molecular Devices. 5 mP standard deviation at 1. The system had to pass vibration load, zero gravity, and temperature fluctuation testings. Description: The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research. The new Molecular Devices instrument comes with a standard 1-year depot warranty service. SpectraMax® iD5 Multi-Mode Microplate Reader - Five-Mode Hybrid Microplate Reader with Automatic NFC Filter Identification and Western Blot Capability, Molecular Devices. Products : FlexStation 3 reader, SpectraMax M5/M5e reader, SpectraMax M2/M2e reader. The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. Find regional support. The optical systemsSpectraMax L: 36. 508/20. 5 mP standard deviation at 1 nMF1 SpectraMax Plus384andABSPlus: Certificate Value – 2≤ Average Peak Value≤ Certificate Value + 2 SpectraMax M2,M2e,M3,M4,M5,andM5e: Certificate Value – 3≤ Average Peak Value≤ Certificate Value + 3 Photometric Accuracy F2, F3, F4 AverageOD=Certificate Value± (Certificate Value(0. 4, Z’ = 0. SpectraMax® M5. A suspicious death, an upscale spiritual retreat, and a quartet of suspects with a motive for murder. SpectraMax M5e: 118. Danaher Inc multi detection microplate reader spectra max m5e Multi Detection Microplate Reader Spectra Max M5e, supplied by Danaher Inc, used in various techniques. (PRWEB) July 19, 2016 -- Molecular Devices, LLC, a leader in protein and cell biology technologies, today announced the launch of the SpectraMax. How do I do this? Note: Check the Applies to section at the bottom of the page to make this is the. 97 cAMP Detection AssayThe SpectraMax M5e reader offers the additional benefit of being certified . No disponible por el momento. This document is provided to customers who have purchased Molecular Devices, Inc. 3. 2222 Fifth Street. The Molecular Devices SpectraMax® M5/M5e Microplate Reader was tested for compatibility with Life Technologies GeneBLAzer® assays. SpectraMax ® ® M5e readers must be appropriately configured for HTRF ® readout by setting up the measurement conditions in the SoftMax Pro software. 40266 Email: [email protected]. Conquer Scientific provides laboratories around the world high quality, low-cost pre-owned laboratory instruments. The SpectraMax® Mini Multi-Mode Microplate Reader offers a budget-friendly solution with three modes of detection for UV-Vis absorbance, fluorescence, and luminescence. With the cAMP dynamic 2 kit, cAMP levels are detected using a Eu. com A. These settings are also valid for the SpectraMax M3/M4, FlexStation® 3 Multi-ModeMulti-mode Microplate Readers. LMax and LMax II plate readers use photon counting mode similar to the SpectraMax L reader. 00We would like to show you a description here but the site won’t allow us. SpectraMax M3, M4, M5, and M5e Microplate Reader User Guide Cuvette Chamber The cuvette chamber is located to the right of the control panel. The large analog PMT works with monochromators and is used during fluorescence. Upgrades to the original platform include temperature control and the ability to configure every aspect of the researcher’s experiment fromM3-M5e_manual. The Analyst®, FlexStation® and SpectraMax® M5/M5e from Molecular Devices have received the LanthaScreen® Certified designation from Invitrogen™ which ensures that these readers are validated to strict standards in instrument seSpectramax i3x multi-mode detection platformMolecular devices spectramax m5e manual I3x spectramax microplate reader multi mode molecular devices minimax cytometer imaging selectscienceSpectramax i3 injury biomechanics medicine materials center. Molecular Devices multi-mode microplate reader, mod SpectraMax M5e, s/n MVE05702 [East Research Lab] Auction Details Bio-Based Mushroom Leather Manufacturing Facility. 1. SpectraMax ABS/ABS Plus SpectraMax i3/i3x MiniMax SpectraMax iD3/iD5 Spectramax 190 EXT Spectramax 340PC/384 Spectramax Plus 384 Spectramax L Spectramax M2 Spectramax M2e Spectramax M3 Spectramax M4 Spectramax M5 Spectramax M5e Spectramax Mini Versamax EXT Vmax Microplate Washers;. Toxicokinetics studies on cynomolgus monkey. The SpectraMax M5e reader offers the additional benefit of being certified . Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. Endpoint, kinetic, spectrum. D 1 1. G-protein coupled receptors signal through two major pathways, regulation of cAMP and increase in intracellular calcium levels mediated by IP3. This document is provided to customers who have purchased Molecular Devices, Inc. Upgrades to the original platform include temperature control and the ability to configure every aspect of the researcher’s experiment fromMolecular Devices SpectraMax™ M5e Microplate Reader w/ Warranty (Svc Contract Available) $34,000. Examples: “Press carefully to engage,” “Do not. 5. Cellular Imaging Systems: Molecular Devices offers various imaging solutions for your research needs. Time Resolved Fluorescence: Solid white plates for top reads and white plates with clear bottoms for bottom reads. 313 reference gain check fail. SpectraMax Mini: 17. Ferguson, Ph. iD3, iD5, i3, i3x, M2, M2e, M3, M4, M5, M5e, FlexStation 3, Gemini EM, and Gemini XPS readers. Homogeneous Time-Resolved Fluorescence (HTRF) . 1. It features a dual-monochromator system, a large dynamic range, and a high-throughput capability. The measurements were performed in triplicate. Categories:. Our multi-mode plate readers provide great flexibility and include absorbance, fluorescence and luminescence with configurable options for fluorescence polarization (FP), time-resolved fluorescence (TRF), FRET, and AlphaScreen. The SpectraMax M5e reader offers the additional benefit of being certified for Cisbio Bioassays HTRF® technology. SpectraMax Mini: 17. LMax, SpectraMax ®, VersaMax™, VMax , EMax , UVMax, FlexStation®, and ThermoMax benchtop Microplate Readers. 0200-7027. SPECTRAmax 340PC 384 Microplate Spectrophotometer Operator’s Manual vii Conventions Used in this Manual The names of keys that appear on the SPECTRAmax PLUS 384 control panel are shown in boxed Helvetica type. the performance of the Molecular Devices SpectraMax® M5e Multi-Mode Microplate Reader with an integrated 8-channel or 16-channel pipettor into one compact benchtop reader. 00 USD. Italic and boldface type are used for emphasis. Configure your microplate reader. SpectraMax M5e Reader only Certified to Cisbio Bioassays’ HTRF technology performance specifications Fluorescence Polarization Performance (M5/M5e only) Wavelength range 300–750 nm Wavelength selection Monochromators, tunable in 1. 7°C (gradient heating design). In this application note we show how the SpectraMax ® i3, SpectraMax ® Paradigm ®, and SpectraMax ® M5e Multi-Mode Microplate Readers are used to perform robust, high-throughput HTRF ® assays with excellent Z’ factors and highly reproducible EC 50 values. Usage/Application: Lab. SpectraMax M5e. 2 x 10-14. , 19 May 2011. 5 mP standard deviation at 1 nMOEM: Molecular DevicesMaterial: SpectraMax M5e Common Name: Microplate Spectrophotometer Item Weight: 36 Serial Number: MVE06577 SKU#: Inv-00352193. The SpectraMax ® M5e Multi-Mode Microplate Reader is a dualmonochromator capable of fluorescence, time-resolved fluorescence (TRF), fluorescence polarization, absorbance, and luminescence assays. The dual monochromator optics allow the widest range of applications to be utilized for bioresearch and drug. Note: In this user guide, SpectraMax ® refers to several SpectraMax . Tailored to your different needs, these kits are configured and optimized for Molecular Devices SpectraMax® microplate readers with preconfigured protocols provid-SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A 5 1. Assay used: ATPlite 1step Luminescence Assay System (PerkinElmer cat. HTRF is a versatile technology developed by Cisbio Bioassays for detecting. measured using a microplate reader (SpectraMax M5e, Molecular Devices, San Jose, CA, USA) with excitation and emission at 494 and 520 nm, respectively, and calibrated against a standard curve for FITC. In the M2, M5 and Plus 384, the light. Wide range of applications. The following day, calcium content was quantified by the QuantiChrom™ Calcium Kit (BioAssay Systems) by reding absorbance at 612 nm using the Spectramax M5e reader (Molecular Devices). #6016736) ATP standards ranging from 1 µM to 1 pM were prepared as. SoftMax Pro GxP is a special software release that includes full user and permission con-Automated Functions Autofocus, autoLED intensity, auto-exposure Autofocus Method Image-based autofocus User-trained autofocus Laser autofocus (option)The SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. For a complete list of the instruments supported by this release of the SoftMax Pro Software, see Supported Instruments on page 111. The dual monochromator optics allow the widest range of applications to be utilized for bioresearch and drug discovery applications, all without the need to change. 1. Products : SoftMax Pro GxP Software, SoftMax Pro Software, SpectraMax M5/M5e reader. SpectraMax i3/i3x and Paradigm readers can be equipped with the HTRF detection cartridge. If not, our scientific teamwill help you choose the instrument that best suits your needs and budget. 8. Products : SpectraMax M2/M2e reader, SpectraMax M3 reader, Gemini EM reader, SpectraMax M5/M5e reader, FlexStation 3 reader, SpectraMax M4 reader Which is better: top-read or bottom-read? You can use either. Homogeneous Time-Resolved Fluorescence (HTRF) . This integrated system provides users with a multi-detection platform capable of increasing the liquid handling throughput and flexibility for biochemical- and cell. Annual recertification of your validation plates ensure that they meet specifications and give you confidence in the performance of your instrument. The M5 has two PMTs. Description 1. Relative fluorescence units (RFU) were converted to units of NADPH concentration (μmol/L) using a standard curve of NADPH. The SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. Description: The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research. book Page 1 Wednesday, July 7, 2010 2:09 PM. 1. 2 x 10-14. Proceed with the following steps using optimized settings. E3300S/L) can be found on the New. 00. Supplier: Molecular Devices. SpectraMax M3, M4, M5, and M5 e Multi-Mode Microplate Readers 0112-0115 F 9 PathCheck ® Pathlength Meas urement Technology A SpectraMax Multi-Mode Microplate Reader with PathCheck Pathlength Measurement Technology allows normalization of variable well volumes to 1-cm cuvette readings. The Analyst®, FlexStation® and SpectraMax® M5/M5 e from Molecular Devices have received the LanthaScreen® Certified designation from Life Technologies which ensures that these readers are validated to strict standards in instrument setup and assay performance. e. Introduction Inflammation, the body’s reaction to infection, irritation, or other injury, is accompanied by increased endothelial chemokine. We would like to show you a description here but the site won’t allow us. 1. The Quant-iT RiboGreen RNA Assay Kit, when run on a SpectraMax microplate reader with fluorescence detection mode and SoftMax Pro Software, is a rapid, sensitive detection method for RNA. 1. Multi-Mode Microplate Readers. M3-M5e_manual. LMax and LMax II RLUs are proportional to (counts/sec) x (integration time), so for these readers if the signal is constant, doubling the integration time doubles the RLU. Path Check Pathlength Measurement We would like to show you a description here but the site won’t allow us. C. SpectraMax Glo and DuoLuc. Conquer Scientific provides laboratories around the world high quality, low-cost pre-owned laboratory instruments. On SpectraMax L, 1 RLU = 1 count per second for photon counting mode only. The Molecular Devices SpectraMax M5 is a multi-mode plate reader that delivers single mode reader performance with five preferred detection modes for a wide range of applications. Twelve different automated tests are provided: • Fluorescence lower limit of detection (LLD) • Excitation wavelength accuracy • Emission wavelength accuracy • Excitation wavelength precisionSpectraMax® M5e Multi-Mode Microplate Reader performance with an integrated 8- or 16-channel pipettor into one compact benchtop reader. Features. It was also read on the monochromator-based SpectraMax® M5e Multi-Mode Microplate Reader using optimized wavelengths. Jul 13, 2023 10am PT Date Format. SpectraMax® M3, M4, M5, and M5e Multi-Mode Microplate Readers User Guide 0112-0115 F July 2010 M3-M5e_manual. The SpectraMax ® M3, M4, M5, and M5 e Microplate Readers are a series of dual-monochromator, multidetection, multi-mode instruments with a triple-mode cuvette port. 5, Z’ = 0. For problems with SpectraMax Multi-Mode Microplate Readers that are not listed here, in the U. 1. The corrected absorbance is within 5% of the. Moving a SpectraMax Multi-Mode Microplate Reader. SpectraMaxi3,M3,M4,M5,M5e,andFlexStation 3instruments G03 through G08forSpectraMaxLinstrument Cross-talk D09 through D11,E09 through E11,andF09 through F11 Results obtained with SpectraMax iD5 (red), i3x (green), and M5e (blue) readers were plotted using a 4-parameter logistic, and EC 50 values of 0. -- JULY 19, 2016Cables. The guide also covers the software features, the specifications, and the accessories of the systems. Products : SpectraMax M4 reader, FilterMax F5 reader, SpectraMax Paradigm reader, SpectraMax M5/M5e reader Do you know any applications for TRF/HTRF that differ from Fluorescence Polarization? TR-FRET assays are well-suited for protein:protein interactions. AVALANCHE. combines Molecular Devices’ SpectraMax® M5e Microplate Reader performance with an integrated 8- or 16-channel pipettor into one compact benchtop reader. The SpectraMax® M5e includes htrf certification, and both the M5 and M5e are lanthascreen certified. 3. D 1 1. The Molecular Devices SpectraMax M5 Multi-Mode Microplate Reader and computer with preload software (StakMax Microplate Handling System/racks not included). Purchase of Additional 1 Year Service Plan includes: MRC Essential Service Plan, including parts, labor, and shipping/handling costs for emergency repairs. Assay used: ATPlite 1step Luminescence Assay System (PerkinElmer cat. Results summary for cAMP HiRange standard curve Figure 3. The Analyst®, FlexStation® and SpectraMax® M5/M5 e from Molecular Devices have received the LanthaScreen® Certified designation from Life Technologies which ensures that these readers are validated to strict standards in instrument setup and assay performance. 304 signal level saturation. Our absorbance plate readers feature our PathCheck Sensor. SpectraMax L: 36. The SpectraMax® M5e includes htrf certification, and both the M5 and M5e are lanthascreen certified. SpectraMax M5eMulti-Mode Microplate Reader (Molecular Devices cat. The resulting purple crystals were dissolved in solubilization buffer and analyzed by spectrophotometry at 490 nm, using a reference of 650 nm in a microplate reader (SpectraMax M5e, MD, USA. Venue Address. Using. A two-fold dilution series of JQ1 (in DMSO) starting from 1 μM was prepared. Cyclic AMP titration curves were evaluated using SpectraMax Paradigm and SpectraMax M5e instruments. SpectraMax M5e Reader only Certified to Cisbio Bioassays HTRF technology performance specifications Fluorescence Polarization Performance (M5/M5e only) Wavelength range 300–750 nm Wavelength selection Monochromators, tunable in 1. For wavelengths in the UV range above 220 nm,. The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance and can be equipped to read volumes as low as 2uL in one multimode reader package. 5. dual monochRomatoRs foR assay flexibility With SpectraMax Multi-Mode Readers, there is no need to utilize expensive filters to optimize detection levels and background. Note: In this user guide, SpectraMax ® refers to several SpectraMax . 5. 312 gain calibration failed. Purchase of Additional 1 Year Service Plan includes: MRC Essential Service Plan, including parts, labor, and shipping/handling costs for emergency repairs. Add to Cart. Choose from a three- (M3), four- (M4), orMicroplate handling system for the spectramax paradigm multi-mode detection platform (110 pages). reader (FI-FLRH) SpectraMax Paradigm. Upgrades to the original platform include temperature control and the ability to configure every aspect of the researcher’s. com A. The Molecular Devices SpectraMax M5E is a multi-mode plate reader that delivers single mode reader performance with five preferred detection modes for a wide range of applications. SUNNYVALE, Calif. Introduction. 1, right panel), for rapid, real-time high throughput screening of mitochondrial (dys) function. Molecular Devices SpectraMax M5e User Manual (86 pages) Multi-Mode Microplate Readers. 1 19. Category 2 and 3 microplate readers use gradient heating as well. Reads various kinds of 96-wells plates, and is equipped with shaking function. The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance and can be equipped to read volumes as low as 2uL in one multimode reader package. These settings are also valid for the SpectraMax M3/M4, FlexStation® 3 Multi-Mode Multi-mode Microplate Readers. The SpectraMax M2 and M2e systems provide the multi detection microplate readers with dualmonochromators, dual-mode cuvette ports, with top & bottom reading. The SpectraMax iD5 reader uses the HTRF detection system, which includes an Enhanced TRF Module and filters for excitation and emission. SpectraMax Mini: 17. This user guide provides detailed instructions on how to operate, maintain, and troubleshoot the. Nanoracks’ Plate-Reader-2 (2nd generation) is a reconfigured Molecular Devices SpectraMax M5e – one of the most reliable, durable, feature-rich microplate readers on the market. SpectraMax L Microplate Reader is a sensitive luminometer with programmable injector options that measures flash and glow luminescence assays, including dual luciferase reporter gene, G protein-coupled receptor (GPCR) in 96- and 384-well plates. Molecular Devices SpectraDrop ™ Micro-Volume Microplate allows users to read up to 64 samples per plate on SpectraMax ® Microplate Readers, with sample volumes as low as 2 µL. Luciferase reporter assays are widely used because of their sensitive detection capacity and broad dynamic range. #M5e) Methods Cell preparation. Detection modes include: • Endpoint, kinetic, spectral and well-scanning • Comprehensive data analysis • Robotics compatibility • Fluorescence Intensity (FI) • Time-Resolved Fluorescence (TRF) The SpectraMax M5e Reader offers the additional benefit of being certified for Cisbio Bioassays’ HTRF® technology. 6 x 10-15. No special upgrade is required for HTRF ® readout, as it is a monochromator-based instrument. The dual monochromator optics allow. 1. 28 nM to 0. book Page 1 Wednesday, July 7, 2010 2:09 PM FlexStation® 3 Benchtop Multi-Mode Microplate Reader User Guide 0112-0127 B June 2013 FlexStation_manual. The SpectraMax® absorbance spectrophotometers and plate readers provide the versatility and convenience for a wide range of assays such as ELISAs, nucleic acid and protein quantitation, and microbial growth. -- JULY 19, 2016 Cables. The SpectraMax M5e Multi-Mode Microplate Reader features a triple-mode cuvette port, temperature control, microplate shaking, and the industry leading SoftMax Pro. 8. Products : SpectraMax M2/M2e reader, SpectraMax M5/M5e reader, Gemini XPS reader, FlexStation II (Obsolete), Gemini EM reader, FlexStation 3 reader Fluorescence Microplate Reader Comparison Chart Features We would like to show you a description here but the site won’t allow us. The SpectraMax® 190 spectrophotometer is ideal for most life science applications, especially DNA analysis. NanoRacks LLC officials have chosen Molecular Devices’ SpectraMax M5e Multi-Mode Microplate Reader for use in NASA's International Space Station. The schematic of the UED setup is shown as Fig. This is a used Molecular Devices SpectraMax Gemini XS Microplate Fluorometer. Dual monochromators for assay flexibility With SpectraMax M Series readers, there is no need to utilize expensive filters to optimize detection levels and background. Molecular Devices recommends that you use high-quality, doubleshielded cables to connect your SpectraMax. The validation kit included individual tubes of diluted fluorophores: FAM, HEX, and FAM+HEX. After the firefly luciferase was measured, Dual-Glo Stop & Glo substrate solution (25 µL/well) was added offline to each well to quench the firefly luciferase reaction and provide substrate for the Renilla luciferase reaction. Detection cartridge modularity allows for easy addition of new read modes within minutes without. The Molecular Devices Gemini XS Fluorescent Microplate Reader lets you experiment with a variety of fluorophores and can even find the best excitation and emission wavelengths for new assays. 8 x 10-15. 6 x 10-15. Cet appareil ISM est confomre à la norme NMB-001 du Canada. 现有酶标仪的客户基本都是需要进行数据分析，可能习惯使用Excel、Prism等软件，对我们SoftMax Pro的数据分析功能不了解，事实上大家可使用SoftMax Pro对数据进行完整处理并不需要再导入第三方. spr * SpectraMax i3(x) SpectraTest ABS2 v1. The SpectraMax® M2 and M2e readers provide two detection modes in one platform, and offer the the added functionality of bottom-read fluorescence for cell-based assays (M2e). By using PathCheck, SpectraMax readers can automatically normalize sample absorbance values to a 1-cm pathlength and calculate the concentrations. 1. iD3, iD5, i3, i3x, M2, M2e, M3, M4, M5, M5e, FlexStation 3, Gemini EM, and Gemini XPS readers. 01 1 100 10000 1000 2000 3000 SpectraMax Paradigm SpectraMax M5e [cAMP] nM delta F % EC 50 Values Paradigm = 18nM M5e = 19nM Variation of Integration Time-12 -10 -8 -6 -4 5000 10000 15000 20000 25000 50 s. The SpectraMax® iD3 Multi-Mode Microplate Reader is the cornerstone of a complete laboratory solution to help you expand the boundaries of your research capabilities. If you need to relocate a SpectraMax Multi-Mode Microplate Reader, follow these steps. 사용이 편리하고 견고한 생명과학 연구, 신약 개발, 개발, Bioassay용 assay. com. 8 x 10-15. View the Molecular Devices website. The SpectraMax® M5e Multi-Mode Microplate Reader delivers single-mode reader performance in one multi-mode reader package. Recommended Optics Wavelength (nm) Wavelength selection Excitation 405/9 Monochromator Emission 1 450/15. Marca: Molecular Devices. Upgrades to the original platform include temperature control and the ability to configure every aspect of the researcher’s experiment fromM3-M5e_manual. SpectraMax M5e: 118. 1. The tunable SpectraMax M5e is a monochromator-based reader developed in collaboration with Cisbio international and is certified to run HTRF® assays. 1, left panel) and individual wells (Fig. 0112-0126B 8 Chapter1:Introduction TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate. The corrected absorbance is within 5% of the value. This version of Molecular Devices SpectraMax M3 Manual compatible with such list of devices, as: SpectraMax M3, SpectraMax M4, SpectraMax M5, SpectraMax M5eModel: SpectraMax™ M5e. Molecular Devices. 2600A. With three modes of detection for UV-Vis absorbance, fluorescence and luminescence, the SpectraMax Mini reader is user upgradeable - from 2 to 3 modes, providing researchers with a versatile instrument that. 1. Labsystems Fluoroskan Ascent FL Microplate Fluorometer. SpectraMax Gemini, SpectraMax I series, SpectraMax M series, FlexStation 3. The SpectraMax®M3, M4, M5, and M5eMicroplate Readers are a series of dual-monochromator, multidetection, multi-mode instruments with a triple-mode cuvette port. #6016736) ATP standards ranging from 1 µM to 1 pM were prepared as. Delivers single mode reader performance in one multimode reader package. The NanoRacks Plate Reader-2 is a reconfigured SpectraMax M5e, one of Molecular Devices’ most reliable, durable, and feature-rich microplate readers, and has been configured to operate comfortably in the microgravity environment of the space station. GE AKTA™ Crossflow. Wide. The Analyst®, FlexStation® and SpectraMax® M5/M5 e from Molecular Devices have received the LanthaScreen® Certified designation from Life Technologies which ensures that these readers are validated to strict standards in instrument setup and assay performance. The donor and acceptor can be used to label a variety of biomolecules, for applications. Sample Fluorescent Reader Setting for the ADP Hunter™ Plus (Cat. Description 1. reader (TUNE) SpectraMax M5e. Bioz Stars score: 86/100, based on 1 PubMed citations. Two cynomolgus monkeys (1 male and 1 female) weighing 2–4 kg and 3–5 years old were purchased from Suzhou. Figure 2 shows an increased expression of the improved. SpectraMax L: 36. Assay used: ATPlite 1step Luminescence Assay System (PerkinElmer cat. application or budget. The Molecular Devices SpectraMax® M5/M5e Microplate Reader was tested for compatibility with Life Technologies Omnia® Assays. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. e. 2 x 10-14. AlphaScreen and AlphaLisa detection using a 680 nm excitation laser diode and a 570/100 nm emission filter. Recommended Optics Wavelength (nm) Wavelength selection Excitation 405/9 Monochromator Emission 1 450/15 Monochromator SpectraMax M5e Reader only Certified to Cisbio Bioassays’ HTRF technology performance specifications Fluorescence Polarization Performance (M5/M5e only) Wavelength range 300–750 nm Wavelength selection Monochromators, tunable in 1. The new Molecular Devices instrument comes with a standard 1-year depot warranty service. Jeffrey Manber, Managing Director of NanoRacks said, "Having the SpectraMax M5e Reader on-orbit will allow researchers to undertake research with real-time results. 6 x 10-15. The chamber contains springs that position the cuvette in the proper alignment for a read. SpectraMax® L (software version 5. Microplate Reader, For Lab. #6016736) ATP standards ranging from 1 µM to 1 pM were prepared as. For aqueous solutions, our patented* PathCheck® sensor technology senses the depth of the liquid in each microplate well, and normalizes the absorbance value to a 1-cm pathlength. INTRODUCTION The SpectraMax M2 and SpectraMax M2 e Multidetection Readers are monochromator based microplate readers that have 6well to 38 4well microplate reading capability as well7. The Analyst®, FlexStation® and SpectraMax® M5/M5e from Molecular Devices have received the LanthaScreen® Certified designation from Invitrogen™ which ensures that these readers are validated to strict standards in instrument seFor calcium content quantification, separate plates were used. Following the user manual for installation, maintenance, assembly or troubleshoot is an crucial prerequisite for safety in addition to protect consumers from any liability from the manufacturer of the product for viable defects in case of an insurance claim. SpectraMax M5e: 118. AlphaScreen assays cannot be done on the SpectraMax M series, Gemini, and SpectraMax iDx (iD3/iD5) readers. 多功能酶标仪 FlexStation3 快速动力学操. 0112-0115G October2018 SpectraMax®M3 SpectraMax®M4 SpectraMax®M5 SpectraMax®M5e Multi-ModeMicroplateReaders UserGuide0112-0115G October2018 SpectraMax®M3 SpectraMax®M4 SpectraMax®M5 SpectraMax®M5e Multi-ModeMicroplateReaders UserGuideGeneration of NADPH was monitored by fluorescence (Ex340/Em460 nm) every minute for 15 minutes on a SpectraMax M5e plate reader (Molecular Devices at 25°C). Skatron Skanwasher 300 Washer. We also buy, refurbish, and resell used lab equipment. 0 nm increments Bandwidth (EX, EM) 9 nm, 15 nm Optimized Precision ≤ 3. the SpectraMax M5e, one of Molecular Devices most reliable and feature-rich microplate readers, to operate comfortably in the zero-gravity environment of the space station. 00 USD. When the user applies a set temperature of 37°C, the top is heated to 37. 5. Data collected by Analyst® and FLIPR® Systems can also be imported and analyzed in the software. 1. 2 x 10-14. After The Plate Is Read, Data Will Appear In The Plate Section: 11. Download Operation & user’s manual of Molecular Devices SpectraMax M3 Laboratory Equipment for Free or View it Online on All-Guides. SpectraMax M5e Reader only Certified to Cisbio Bioassays’ HTRF technology performance specifications Fluorescence Polarization Performance (M5/M5e only) Wavelength range 300–750 nm Wavelength selection Monochromators, tunable in 1. The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. The SpectraMax M5e Multi-Mode Microplate Reader features a triple-mode cuvette port, temperature control, microplate shaking, and the industry-leading SoftMax Pro. 8 x 10-15. Microplate Readers. SpectraMax M2. 3. M5e: W = 15. The SpectraMax M5e Multi. Optimal instrument settings are the same for all three readers. Reference and the Water Constant methods. Features. Microplate Reader Shelf. Meso Scale Discovery 1250 Sector Imager 2400 $ 2,500. The SpectraMax® M3, M4, M5 and M5e readers offer a modular, upgradeable dual monochromator microplate reader platform with a wide range of high performance multi. Both instruments demonstrated good performance for the assay. Configuring the SpectraMax M5, M5e, and FlexStation 3 Multi-Mode Microplate Readers for ValitaTiter Assays. for Cisbio Bioassays HTRF® technology. 1. This assay may also be detected on the SpectraMax ® i3 and SpectraMax ® M5e Multi-Mode Microplate ReadersThe SpectraMax M5e reader offers the additional benefit of being certified for Cisbio Bioassays HTRF® technology. The BRD4 bromodomain 1 TR-FRET assay was performed as described in the assay kit manual.